Journal: American Journal of Cancer Research

Article Title: The ADAM9/UBN2/AKR1C3 axis promotes resistance to androgen-deprivation in prostate cancer

doi:

Figure Lengend Snippet: List of antibodies used in the study

Article Snippet: To normalize the secreted protein among conditioned media samples, the loading amount was adjusted to represent the same quantity of cell numbers (10 5 cells), and ponceau S stain of the nitrocellulose membranes was used to confirm equal levels of protein input. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Source CAT# Anti-Active integrin β 1 Merck, Darmstadt, Germany MAB2079Z Anti-ADAM9 (for IHC) Merck, Darmstadt, Germany HPA004000 Anti-ADAM9 (for WB) R&D systems, Minneapolis, MN, USA AF939 Anti-AKR1C3 Merck, Darmstadt, Germany A6229 Anti-AKT Cell signaling technology, Danvers, MA, USA 9272 Anti-AR (N-20) Santa Cruz Biotechnology, Dallas, TX, USA SC-816 Anti-EF1α Antibody, clone CBP-KK1 Merck, Darmstadt, Germany 05-235 Anti-FAK Cell signaling technology, Danvers, MA, USA 3284 Anti-Integrin β 1 Merck, Darmstadt, Germany MABT529 Anti-pAKT (Ser473) Cell signaling technology, Danvers, MA, USA 9271 Anti-pFAK (Ser 397) and pFAK (Ser 576/577) Cell signaling technology, Danvers, MA, USA 3283 and 3281 Anti-PSA Santa Cruz Biotechnology, TX, USA SC-7636 Anti-UBN2 (for IHC) Merck, Darmstadt, Germany HPA019743 Anti-UBN2 (WB) ProSci, Fort Collins, CO, USA 57-094 Anti-β-actin GeneTex, Irvine, CA, USA GTX109639 Donkey Anti-Goat IgG (H+L) Jackson Immuno Research, West Grove, PA, USA 705-215-147 Donkey Anti-mouse IgG (H+L) Jackson Immuno Research, West Grove, PA, USA 715-195-150 Donkey Anti-rabbit IgG (H+L) Jackson Immuno Research, West Grove, PA, USA 711-005-152 Open in a separate window List of antibodies used in the study RNA extraction and real-time quantitative polymerase chain reaction (qPCR) Total cellular RNAs were isolated with a NC RNA extraction reagent from EBL Biotechnology (New Taipei City, Taiwan).

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